B100 yes, regular#2 no. The higher the content of biodiesel the higher the temp at which it begins to gel. Cold climates drop B20 in the winter time. I believe B100 gels up within a few degrees of freezing.
Also widely overlooked is the fact that biodiesel raises NOX emissions which is the primary target emission regarding diesel. Europe is just now figuring this out.
OEMs have conceded OCI and fuel filter life are both shortened compared to conventional#2 diesel. In the early days of B20 almost all OEMs required oil change intervals be cut in half when using B20. I do not know what has changed to alter their position on this but that tells you the stuff just isn't as good as Uncle Sam would like you to believe. Also the potential is much greater for contamination in bulk storage or in vehicles that do not use fuel on a regular basis. It is not recommended in stand by generators for this reason.
Lastly, all current HPCR engines are designed to run off of #2 diesel. No "extra" lube required.
I realize many do not have the option, but I can not understand why anyone would choose to run higher concentrations if a choice is available. I've seen first hand the mess the stuff creates in my cousin's farm tanks. Keep in mind he averages over 1000 gallons a month. By the time it was all said and done he was out thousands of dollars- shocking, cleaning, tank draining, multiple filter changes and repeated down time. No thank you.